Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase of Schistosoma japonicum.
Identifieur interne : 000251 ( Main/Exploration ); précédent : 000250; suivant : 000252Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase of Schistosoma japonicum.
Auteurs : Li-Jun Song [République populaire de Chine] ; Jia-Huang Li [République populaire de Chine] ; Xu-Ren Yin [République populaire de Chine] ; Wei Zhang [République populaire de Chine] ; Yi Jin [République populaire de Chine] ; Hong Gao [République populaire de Chine] ; Jie Wang [République populaire de Chine] ; Chuan-Xin Yu [République populaire de Chine] ; Zi-Chun Hua [République populaire de Chine]Source :
- BioMed research international [ 2314-6141 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Complexes multienzymatiques, NADH, NADPH oxidoreductases.
- enzymologie : Schistosoma japonicum.
- Animaux, Glutathione reductase, Peptides, Simulation numérique.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Multienzyme Complexes, NADH, NADPH Oxidoreductases.
- chemical : Glutathione Reductase, Peptides.
- enzymology : Schistosoma japonicum.
- Animals, Computer Simulation.
Abstract
Schistosomiasis is one of the world's major public health problems. Praziquantel is currently the only effective drug against schistosomiasis. As resistance of praziquantel has emerged in some endemic areas, development of new antischistosomal agents should be a high priority. In this study, a phage display peptide library was used for screening for peptide antagonists of thioredoxin glutathione reductase of Schistosoma japonicum (SjTGR), which has been identified as an alternative drug target. Three rounds of panning produced four different fusion phages. ELISA proved that all four phages could bind to SjTGR. One peptide, JIPDys1 (aa, WPHNWWPHFKVK), reduced enzyme activity of SjTGR by more than 50%. 2 μM of the synthesized peptide of JIPDys1 inhibited the activity of TrxR, GR, and Grx of SjTGR by 32.5%, 100%, and 100%, respectively. The IC50 values of the synthetic peptide JIPDys1 for TrxR, GR, and Grx were 3.67 μM, 0.11 μM, and 0.97 μM, respectively. Based on computer simulation, it appeared that JIPDys1 binds to the substrate binding sites of glutathione reductase (GR) and glutaredoxin (Grx). Our data show that the peptide, JIPDys1 (aa, WPHNWWPHFKVK), is a promising candidate to develop novel drugs against S. japonicum which acts by binding with SjTGR and reduces enzyme activity of SjTGR.
DOI: 10.1155/2018/9483928
PubMed: 29967790
PubMed Central: PMC6008883
Affiliations:
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xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province 210008, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province 210008</wicri:regionArea><wicri:noRegion>Jiangsu Province 210008</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jie" sort="Wang, Jie" uniqKey="Wang J" first="Jie" last="Wang">Jie Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chuan Xin" sort="Yu, Chuan Xin" uniqKey="Yu C" first="Chuan-Xin" last="Yu">Chuan-Xin Yu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Hua, Zi Chun" sort="Hua, Zi Chun" uniqKey="Hua Z" first="Zi-Chun" last="Hua">Zi-Chun 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213164</wicri:regionArea><wicri:noRegion>Jiangsu Province 213164</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Shenzhen Research Institute of Nanjing University, Shenzhen, Guangdong Province 518057, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shenzhen Research Institute of Nanjing University, Shenzhen, Guangdong Province 518057</wicri:regionArea><wicri:noRegion>Guangdong Province 518057</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29967790</idno><idno type="pmid">29967790</idno><idno type="doi">10.1155/2018/9483928</idno><idno type="pmc">PMC6008883</idno><idno type="wicri:Area/Main/Corpus">000232</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000232</idno><idno type="wicri:Area/Main/Curation">000232</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000232</idno><idno type="wicri:Area/Main/Exploration">000232</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase of <i>Schistosoma japonicum</i>.</title><author><name sortKey="Song, Li Jun" sort="Song, Li Jun" uniqKey="Song L" first="Li-Jun" last="Song">Li-Jun Song</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023</wicri:regionArea><wicri:noRegion>Jiangsu Province 210023</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Li, Jia Huang" sort="Li, Jia Huang" uniqKey="Li J" first="Jia-Huang" last="Li">Jia-Huang Li</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023</wicri:regionArea><wicri:noRegion>Jiangsu Province 210023</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164</wicri:regionArea><wicri:noRegion>Jiangsu Province 213164</wicri:noRegion></affiliation></author><author><name sortKey="Yin, Xu Ren" sort="Yin, Xu Ren" uniqKey="Yin X" first="Xu-Ren" last="Yin">Xu-Ren Yin</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Hong" sort="Gao, Hong" uniqKey="Gao H" first="Hong" last="Gao">Hong Gao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province 210008, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province 210008</wicri:regionArea><wicri:noRegion>Jiangsu Province 210008</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jie" sort="Wang, Jie" uniqKey="Wang J" first="Jie" last="Wang">Jie Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chuan Xin" sort="Yu, Chuan Xin" uniqKey="Yu C" first="Chuan-Xin" last="Yu">Chuan-Xin Yu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064</wicri:regionArea><wicri:noRegion>Jiangsu Province 214064</wicri:noRegion></affiliation></author><author><name sortKey="Hua, Zi Chun" sort="Hua, Zi Chun" uniqKey="Hua Z" first="Zi-Chun" last="Hua">Zi-Chun Hua</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023</wicri:regionArea><wicri:noRegion>Jiangsu Province 210023</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164</wicri:regionArea><wicri:noRegion>Jiangsu Province 213164</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Shenzhen Research Institute of Nanjing University, Shenzhen, Guangdong Province 518057, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shenzhen Research Institute of Nanjing University, Shenzhen, Guangdong Province 518057</wicri:regionArea><wicri:noRegion>Guangdong Province 518057</wicri:noRegion></affiliation></author></analytic><series><title level="j">BioMed research international</title><idno type="eISSN">2314-6141</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Computer Simulation (MeSH)</term><term>Glutathione Reductase (MeSH)</term><term>Multienzyme Complexes (antagonists & inhibitors)</term><term>NADH, NADPH Oxidoreductases (antagonists & inhibitors)</term><term>Peptides (MeSH)</term><term>Schistosoma japonicum (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Complexes multienzymatiques (antagonistes et inhibiteurs)</term><term>Glutathione reductase (MeSH)</term><term>NADH, NADPH oxidoreductases (antagonistes et inhibiteurs)</term><term>Peptides (MeSH)</term><term>Schistosoma japonicum (enzymologie)</term><term>Simulation numérique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Multienzyme Complexes</term><term>NADH, NADPH Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glutathione Reductase</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Complexes multienzymatiques</term><term>NADH, NADPH oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Schistosoma japonicum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Schistosoma japonicum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Computer Simulation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glutathione reductase</term><term>Peptides</term><term>Simulation numérique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Schistosomiasis is one of the world's major public health problems. Praziquantel is currently the only effective drug against schistosomiasis. As resistance of praziquantel has emerged in some endemic areas, development of new antischistosomal agents should be a high priority. In this study, a phage display peptide library was used for screening for peptide antagonists of thioredoxin glutathione reductase of <i>Schistosoma japonicum</i> (SjTGR), which has been identified as an alternative drug target. Three rounds of panning produced four different fusion phages. ELISA proved that all four phages could bind to SjTGR. One peptide, JIPDys1 (aa, WPHNWWPHFKVK), reduced enzyme activity of SjTGR by more than 50%. 2 <i>μ</i>M of the synthesized peptide of JIPDys1 inhibited the activity of TrxR, GR, and Grx of SjTGR by 32.5%, 100%, and 100%, respectively. The IC<sub>50</sub> values of the synthetic peptide JIPDys1 for TrxR, GR, and Grx were 3.67 <i>μ</i>M, 0.11 <i>μ</i>M, and 0.97 <i>μ</i>M, respectively. Based on computer simulation, it appeared that JIPDys1 binds to the substrate binding sites of glutathione reductase (GR) and glutaredoxin (Grx). Our data show that the peptide, JIPDys1 (aa, WPHNWWPHFKVK), is a promising candidate to develop novel drugs against <i>S. japonicum</i> which acts by binding with SjTGR and reduces enzyme activity of SjTGR.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29967790</PMID><DateCompleted><Year>2019</Year><Month>01</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>01</Month><Day>07</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">2314-6141</ISSN><JournalIssue CitedMedium="Internet"><Volume>2018</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>BioMed research international</Title><ISOAbbreviation>Biomed Res Int</ISOAbbreviation></Journal><ArticleTitle>Identification of Peptide Antagonists to Thioredoxin Glutathione Reductase of <i>Schistosoma japonicum</i>.</ArticleTitle><Pagination><MedlinePgn>9483928</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1155/2018/9483928</ELocationID><Abstract><AbstractText>Schistosomiasis is one of the world's major public health problems. Praziquantel is currently the only effective drug against schistosomiasis. As resistance of praziquantel has emerged in some endemic areas, development of new antischistosomal agents should be a high priority. In this study, a phage display peptide library was used for screening for peptide antagonists of thioredoxin glutathione reductase of <i>Schistosoma japonicum</i> (SjTGR), which has been identified as an alternative drug target. Three rounds of panning produced four different fusion phages. ELISA proved that all four phages could bind to SjTGR. One peptide, JIPDys1 (aa, WPHNWWPHFKVK), reduced enzyme activity of SjTGR by more than 50%. 2 <i>μ</i>M of the synthesized peptide of JIPDys1 inhibited the activity of TrxR, GR, and Grx of SjTGR by 32.5%, 100%, and 100%, respectively. The IC<sub>50</sub> values of the synthetic peptide JIPDys1 for TrxR, GR, and Grx were 3.67 <i>μ</i>M, 0.11 <i>μ</i>M, and 0.97 <i>μ</i>M, respectively. Based on computer simulation, it appeared that JIPDys1 binds to the substrate binding sites of glutathione reductase (GR) and glutaredoxin (Grx). Our data show that the peptide, JIPDys1 (aa, WPHNWWPHFKVK), is a promising candidate to develop novel drugs against <i>S. japonicum</i> which acts by binding with SjTGR and reduces enzyme activity of SjTGR.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Li-Jun</ForeName><Initials>LJ</Initials><Identifier Source="ORCID">0000-0002-3897-5925</Identifier><AffiliationInfo><Affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jia-Huang</ForeName><Initials>JH</Initials><Identifier Source="ORCID">0000-0003-3513-4159</Identifier><AffiliationInfo><Affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Xu-Ren</ForeName><Initials>XR</Initials><Identifier Source="ORCID">0000-0003-3463-3378</Identifier><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Wei</ForeName><Initials>W</Initials><Identifier Source="ORCID">0000-0001-6791-5043</Identifier><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><Identifier Source="ORCID">0000-0002-2103-9831</Identifier><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Hong</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0002-8518-0033</Identifier><AffiliationInfo><Affiliation>Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province 210008, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jie</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-9453-9642</Identifier><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Chuan-Xin</ForeName><Initials>CX</Initials><Identifier Source="ORCID">0000-0002-6662-3847</Identifier><AffiliationInfo><Affiliation>Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214064, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hua</LastName><ForeName>Zi-Chun</ForeName><Initials>ZC</Initials><Identifier Source="ORCID">0000-0002-4654-1801</Identifier><AffiliationInfo><Affiliation>School of Life Sciences and the State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu Province 210023, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu Province 213164, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Shenzhen Research Institute of Nanjing University, Shenzhen, Guangdong Province 518057, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biomed Res Int</MedlineTA><NlmUniqueID>101600173</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009097">Multienzyme Complexes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.-</RegistryNumber><NameOfSubstance UI="D009247">NADH, NADPH Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.4.-</RegistryNumber><NameOfSubstance UI="C466433">thioredoxin glutathione reductase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.8.1.7</RegistryNumber><NameOfSubstance UI="D005980">Glutathione Reductase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003198" MajorTopicYN="Y">Computer Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005980" MajorTopicYN="N">Glutathione Reductase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009097" MajorTopicYN="N">Multienzyme Complexes</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009247" MajorTopicYN="N">NADH, NADPH Oxidoreductases</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="Y">Peptides</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012549" MajorTopicYN="N">Schistosoma japonicum</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>03</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29967790</ArticleId><ArticleId IdType="doi">10.1155/2018/9483928</ArticleId><ArticleId IdType="pmc">PMC6008883</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Biol Chem. 2010 Oct 15;285(42):32557-67</Citation><ArticleIdList><ArticleId IdType="pubmed">20659890</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Negl Trop Dis. 2009 Aug 18;3(8):e504</Citation><ArticleIdList><ArticleId IdType="pubmed">19688043</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Negl Trop Dis. 2017 Feb 17;11(2):e0005372</Citation><ArticleIdList><ArticleId 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sortKey="Hua, Zi Chun" sort="Hua, Zi Chun" uniqKey="Hua Z" first="Zi-Chun" last="Hua">Zi-Chun Hua</name><name sortKey="Hua, Zi Chun" sort="Hua, Zi Chun" uniqKey="Hua Z" first="Zi-Chun" last="Hua">Zi-Chun Hua</name><name sortKey="Hua, Zi Chun" sort="Hua, Zi Chun" uniqKey="Hua Z" first="Zi-Chun" last="Hua">Zi-Chun Hua</name><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><name sortKey="Li, Jia Huang" sort="Li, Jia Huang" uniqKey="Li J" first="Jia-Huang" last="Li">Jia-Huang Li</name><name sortKey="Li, Jia Huang" sort="Li, Jia Huang" uniqKey="Li J" first="Jia-Huang" last="Li">Jia-Huang Li</name><name sortKey="Song, Li Jun" sort="Song, Li Jun" uniqKey="Song L" first="Li-Jun" last="Song">Li-Jun Song</name><name sortKey="Wang, Jie" sort="Wang, Jie" uniqKey="Wang J" first="Jie" last="Wang">Jie Wang</name><name sortKey="Yin, Xu Ren" sort="Yin, Xu Ren" uniqKey="Yin X" first="Xu-Ren" last="Yin">Xu-Ren Yin</name><name sortKey="Yu, Chuan Xin" sort="Yu, Chuan Xin" uniqKey="Yu C" first="Chuan-Xin" last="Yu">Chuan-Xin Yu</name><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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